ProGT41 (NGT)

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ProGT ID ProGT41 (NGT)
Organism Information
Organism NameActinobacillus pleuropneumoniae
Clinical ImplicationPathogenic
DomainBacteria
Classification Phylum : Proteobacteria
Class : GammaProteobacteria
Orders : Pasteurellales
Family : Pasteurellaceae
Genus : Actinobacillus
Species : pleuropneumoniae
Taxonomic ID (NCBI)715
Genome Information
Gene BankCP022715.1
EMBLCP022715.1
Gene Information
Gene Nameappser1_17560
Protein information
Protein NameNGT 
UniProtKB/ SwissProt IDA0A223MD76
NCBI Ref SeqWP_005599032.1
UniProtKB Sequence>tr|A0A223MD76|A0A223MD76_ACTPL UDP-glucose:protein N-beta-glucosyltransferase OS=Actinobacillus pleuropneumoniae OX=715 GN=CHY23_00708 PE=4 SV=1 MENENKPNVANFEAAVAAKDYEKACSELLLILSQLDSNFGGIQEIEFEYPAQLQDLEQEK IVYFCTRMATAITTLFSDPVLEISDLGVQRFLVYQRWLALIFASSPFVNADHILQTYNRE PNRKNSLEIHLDSSKSSLIKFCILYLPESNVNLNLDVMWNISPELCASLCFALQSPRFIG TSTAFNKRATILQWFPRHLDQLKNLNNIPSAISHDVYMHCSYDTSVNKHDVKRALNHVIR RHIESEYGWKDRDVAHIGYRNNKPVMVVLLEHFHSAHSIYRTHSTSMIAAREHFYLIGLG SPSVDQAGQEVFDEFHLVAGDNMKQKLEFIRSVCESNGAAIFYMPSIGMDMTTIFASNTR LAPIQAIALGHPATTHSDFIEYVIVEDDYVGSEECFSETLLRLPKDALPYVPSALAPEKV DYLLRENSEVVNIGIASTTMKLNPYFLEALKAIRDRAKVKVHFHFALGQSNGITHPYVER FIKSYLGDSATAHPHSPYHQYLRILHNCDMMVNPFPFGNTNGIIDMVTLGLVGVCKTGAE VHEHIDEGLFKRLGLPEWLIANTVDEYVERAVRLAENHQERLELRRYIIENNGLNTLFTG DPRPMGQVFLEKLNAFLKEN
EMBL CDSASU15484
Sequence length620 AA
Subcellular LocationCytoplasm
String228399.appser1_17560
Glycosyltransferase Information
Glycosylation TypeN- (Asn) linked 
CAZY FamilyGT41
EC Number (BRENDA)2.4.1.-
Mechanism of Glycan TransferSequential
Acceptor specificity Sequon_1Asn-Xaa-Ser/Thr
Donor TypeUDP-Glc and UDP-Gal ( UDP-Glc is preferred over UDP-Gal)
Donor SpecificityNucleotide activated sugars (UDP and GDP linked hexose)
Glycan Information
Glycan transferredMonosaccharides (Glc and Gal) 
Method of Glycan IndentificationGlycoProfile III Fluorescent Glycoprotein Detection kit
Experimental_strategiesIn vitro and In vivo  
Acceptor Subtrate Information
Acceptor Substrate name HMW1ct
ProGPdb ID ProGP227
Litrature
Year Of Validation2010 
Reference Choi, K.J., Grass, S., Paek, S., St. Geme III, J.W. and Yeo, H.J., 2010. The Actinobacillus pleuropneumoniae HMW1C-like glycosyltransferase mediates N-linked glycosylation of the Haemophilus influenzae HMW1 adhesin. PLoS One, 5(12), p.e15888.

Corresponding AuthorDepartment of Biology and Biochemistry, University of Houston, Houston, Texas, United States of America
Reference Kawai, F., Grass, S., Kim, Y., Choi, K.J., Geme, J.W.S. and Yeo, H.J., 2011. Structural insights into the glycosyltransferase activity of the Actinobacillus pleuropneumoniae HMW1C-like protein. Journal of Biological Chemistry, 286(44), pp.38546-38557.

Corresponding AuthorDepartment of Biology and Biochemistry, University of Houston, Houston, Texas 77204.
Reference Song, Q., Wu, Z., Fan, Y., Song, W., Zhang, P., Wang, L., Wang, F., Xu, Y., Wang, P.G. and Cheng, J., 2017. Production of homogeneous glycoprotein with multisite modifications by an engineered N-glycosyltransferase mutant. Journal of Biological Chemistry, 292(21), pp.8856-8863.

Corresponding AuthorState Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin 300353, China
Reference Lomino, J.V., Naegeli, A., Orwenyo, J., Amin, M.N., Aebi, M. and Wang, L.X., 2013. A two-step enzymatic glycosylation of polypeptides with complex N-glycans. Bioorganic & medicinal chemistry, 21(8), pp.2262-2270.

Corresponding AuthorInstitute of Human Virology and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, United States
Reference Xu, Y., Wu, Z., Zhang, P., Zhu, H., Zhu, H., Song, Q., ... & Cheng, J. (2017). A novel enzymatic method for synthesis of glycopeptides carrying natural eukaryotic N-glycans. Chemical Communications, 53(65), 9075-9077.

Corresponding AuthorState Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin 300353, China